Construction and Transformation of Plasmid Containing Swollenin Gene for Lentinus edodes

doi:10.7506/spkx1002-6630-201111031

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (11): 141-145.doi: 10.7506/spkx1002-6630-201111031

• Bioengineering • Previous Articles Next Articles

Construction and Transformation of Plasmid Containing Swollenin Gene for Lentinus edodes

LIU Yan1，YAO Qiang2，GONG Zhi-yuan2,*，GAO Xing-xi3，HAN Jian-dong2，REN Peng-fei2，ZHANG Xue-mei1， REN Hai-xia2，LI Jin2，QU Ling2

1. School of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China； 2. Institute of Agricultural Resources and Environment, Shandong Acdemy of Agricultural Sciences, Jinan 250100, China； 3. Institute of Mycological Science and Technology, Ludong University, Yantai 264025, China

Online:2011-06-15 Published:2011-05-13

Abstract

Abstract: Swollenin can loosen cellulose and enhance its enzymatic hydrolysis. An expression plasmid containing swollenin(swo) gene was constructed and successfully transformed to Lentinus edodes by PEG-mediated transformation method with an efficiency of 1 transformant/μg plasmid DNA. PCR and Southern blot indicated that swollenin was successfully integrated to the genome of L. edodes. Both tested transformants of L. edodes can form fruit bodies and transformant II still revealed good cultivation characteristics. Transformation of swo gene into L. edodes can provide an exploration into breeding of new varieties of L. edodes.

Key words: Lentinus edodes, swollenin gene, expression plasmid, genetic transformation

CLC Number:

S646.1

LIU Yan，YAO Qiang，GONG Zhi-yuan，GAO Xing-xi，HAN Jian-dong，REN Peng-fei，ZHANG Xue-mei，REN Hai-xia，LI Jin，QU Ling. Construction and Transformation of Plasmid Containing Swollenin Gene for Lentinus edodes[J]. FOOD SCIENCE, 2011, 32(11): 141-145.

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Construction and Transformation of Plasmid Containing Swollenin Gene for Lentinus edodes

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