Abstract:

This study aimed to compare the composition and antioxidant activity of polysaccharides from spent mushroom
substrates of Agrocybe cylindracea, Lentinus edodes, white Hypsizygus marmoreu, Pleurotus ostreatus and Cordyceps
militaris. The polysaccharides were prepared after enzymatic extraction with cellulase, precipitation with ethanol and
deproteinization with Sevag regent, and then their monosaccharide composition and antioxidant activities were measured.
The results showed that all the polysaccharides had antioxidant activities. Therein, the polysaccharides from spent mushroom
substrate of Agrocybe cylindracea had the best oxygen free radical scavenging capacity and ferric reducing power, with
ORAC value of 1 215 μmol equivalent Trolox/g and FRAP value of 281 μmol equivalent Fe2+/g. The polysaccharides from
spent mushroom substrates of white Hypsizygus marmoreus and Pleurotus ostreatus possessed better ABTS and DPPH
radical scavenging activities than that from other spent mushroom substrates.

Key words: spent mushroom substrates, polysaccharide, antioxidant activity, Agrocybe cylindracea, Lentinus edodes;white Hypsizygus marmoreu, Pleurotus ostreatus, Cordyceps militaris