FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (3): 203-205.

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Gene Clone and Sequence Analysis of Glycerol Dehydratase

 ZHENG  Yan, GUAN  Yi-Fei, LIU  Chang-Jiang   

  1. 1.Food Biotechnology Laboratory, College of Food Science, Shenyang Agricultural University, Shenyang 110161, China; 2.The Government of Liaoning Province, Shenyang 110000, China
  • Online:2007-03-15 Published:2011-12-31

Abstract: The gene of gldABC encoding glycerol dehydratase was amplified by PCR and cloned into the vector of pMD19- T Simple marker. Sequence analysis showed that the gene of gldABC is 2816bp including three open reading frames encoded polypeptides of 556,194 and 142 amino acids respectively. The deduced amino acid sequences homologized with Klebsiella pneumoniae ATCC25955 is 100%.

Key words: K.pneumoniae, 1,3-propanediol, glycerol dehydratase, clone