Synthesis and Identification of Medroxy Progesterone Acetate Artificial Antigen

Abstract

Abstract: Medroxy progesterone acetate(MPA), as a hapten, cannot be led to immune reaction and has to be conjugated with a carrier, such as bovine serum albumin(BSA). The carbodiimides reaction was used to prepare the immunogen in this paper. The conjugates of MPA-BSA were identified by the analysis of UV-spetrum, SDS-PAGE and animal immunization test. An ELISA method was established with MPA antibody, which has the detection limit of 1.8μg/ml and the linear detection of 2.5～ 50μg/ml(r2=0.9936).

Key words: medroxy progesterone acetate(MPA), carbodiimides, artificial antigen, ELISA

SUN Wu-Yong, LI Fei-Fei, QU Ling-Bo, WU Yong-Jun, WANG Yun-Long, LIU Xiang-Hui. Synthesis and Identification of Medroxy Progesterone Acetate Artificial Antigen[J]. FOOD SCIENCE, 2007, 28(3): 274-277.

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Synthesis and Identification of Medroxy Progesterone Acetate Artificial Antigen

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