Molecular Clone and Expression of Nattokinase Gene in Escherichia coli and Bacillus subtilis

Abstract

Abstract: Chromosome DNA was isolated from Bacillus subtilis(natto.var).Pre-pro-nk(encoding signal peptide,propeptide and mature peptide),pro-nk(encoding propeptide and mature peptide)and nk(encoding mature peptide)were then amplified from chromosome by PCR.The expression vector pET28a-nk and pHT315-nk was constructed and transformed into Escheri- chia coli and Bacillus subtilis to actively express nattokinase and be purified through ion-exchange chromatography.

Key words: nattokinase gene, Escherichia coli, Bacillus subtilis, active expression

HUANG Lei, XIE Yu-Juan, LI Shen, LI Dan, LIANG Feng-Lai, LIU Ru-Lin. Molecular Clone and Expression of Nattokinase Gene in Escherichia coli and Bacillus subtilis[J]. FOOD SCIENCE, 2007, 28(5): 199-202.

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Molecular Clone and Expression of Nattokinase Gene in Escherichia coli and Bacillus subtilis

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