Abstract: Aim:To set up a simple,high-speed,and repeatable method used for determining cholesterol content in food samples, to benefit the people to control the intake of cholesterol.Method:The cookie samples were extracted with petroleum ether first, and the extraction was saponified with 50% KOH-ethanol（40:12）solution.Then the saponified solution was extracted again with ether.The upper solution was washed to neutral with distilled water and was dehydrated with anhydrous Na2SO4N.The dehydrated solution was filtered and concentrated to about 1ml and then blowed to dry with N2.The dried remains were dissolved with n-hexane and were quantitatively analyzed with GC-MS.Result:The result showed that with this method,the recovery of cholesterol is about 98.43%;the standard deviation 2.193%,and RSD（n=6）1.4393% with the minimum detection limit 0. 0659μg/ml and the minimum ration 0.22μg/ml.Conclusion:This method can be applied to determine the cholesterol content in different food samples.

Key words: gas chromatography-mass spectrometry, cookie, cholesterol