FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (6): 74-77.

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Primary Study on Bacteriostatic Active Compound from Leaves of Loropetalum chinense var. rubrum Yieh

 ZHOU  Guo-Hai, YU  Hua-Zhong, LU  Cheng-Ying, ZHOU  Zhe   

  1. 1.College of Resources and Planning Sciences, Jishou University, Zhangjiajie 427000, China; 2.Key Laboratory of Hunan Forest Products Chemical Engineering, Zhangjiajie 427000, China
  • Online:2007-06-15 Published:2011-12-31

Abstract: In this paper, the leaves of Loropetalum chinense var. rubrum Yieh were used as raw material, and the bacteriostatic active compound with different solvents extracted in turn. The bacteriostatic effects on Staphylococlus aureus, Shigella dysenteroae and Escheichia colis were studied with bacteriostatic experiment. The bacteriostatic active extract was separated by thin layer chromatography. The best developer is found. Then the active compounds separated by thin layer chromatography were tested by the bacteriostatic experiment and chemical recognition so as to affirm the species of bacteriostasis active compounds. The results showed that ethyl acetate is the best solvent and the best developer solvent is: chloroform: acetone: formic acid: water (60:35:5:27 respectively). The main active compounds in the leaves of Loropetalum chinense var. rubrum Yieh are tannin and coumarin.

Key words: leaves of Loropetalum chinense var. rubrum Yieh, bacteriostasis, active compounds, thin layer chromatography