FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (7): 198-201.

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Optimization of Extraction Technique of Polyonatic Sibiricum Polysaccharides by Response Surface Analysis

 CHEN  Gang, CHEN  Hong-Lan, SU  Wei, ZHANG  Gui-Zhi   

  1. 1.College of Life Science, Nanchang University, Key Laboratory of Food Science, Ministry of Education, Nanchang 330047, China;2.Jiangxi College of Applied Technology, Ganzhou 341000, China; 3.College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China; 4.College of Food Science, Xinjiang Agricultural Univercity, Urumqi 830052, China
  • Online:2007-07-15 Published:2011-10-24

Abstract: In this paper, aiming at optimization of the extraction of Polyonatic Sibiricum polysaccharides (PSP), test factors and levels were firstly selected by one-factor tests. On the basis of previous results and according to the central composite test design principles, the method of response surface method (RSM) with 3 factors and 3 levels was adopted. Response surface and contour are finally graphed with the extraction rate of PSP as the response value. The results showed that the optimum conditions of PSP extraction are: ratio of water to water 21.5:1, the extraction temperature 73.5 ℃, extraction time 2.5 h. The extraction rate of PSP is up to 12.25%, and it is higher 10.6% than one-factor tests.

Key words: Polyonatic Sibiricum polysaccharides, extraction, response surface methodology