Study on Isolation and Purification of Chlorogenic Acid in Flos Lonicerae

Abstract

Abstract: Objecive: To study the isolation and purification conditions of chlorogenic acid(CA) from Flos Lonicerae. Method: The isolation and purification of CA were achieved by polyamide column chromatography and the main isolation factors were optimized. Results: The optimum isolation is as following: the rate of crude CA to polymide is 1:5, the rate of diameter to long is 1:7, 10% alcohol washed at high speed under usual pressure, CA is collected between the volume of 5 ～ 14 times of column volume(A322/A216 ≥0.9), under the conditions, the product with above 70% CA is obtained, which can be further crystalled by EtoAc and the purity is enhanced to 93%. Conclusion: This method is simple, low cost, and suitable to be used in industry.

Key words: Flos Lonicerae, chlorogenic acid, polyamide column chromatography, isolation, purification

YANG Min-Li, HAO Feng-Xia. Study on Isolation and Purification of Chlorogenic Acid in Flos Lonicerae[J]. FOOD SCIENCE, 2007, 28(7): 255-259.

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Study on Isolation and Purification of Chlorogenic Acid in Flos Lonicerae

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