Abstract: Interactive effects of growth medium, cryoprotector and starvation treatment on bacterial survival during the storage of freeze-dried lactic acid bacteria have been studied. Three factors, including addition of NaCl to the growth medium, addition of sucrose to the drying medium and starvation of S-1 in the stationary phase, could help survive during storage in the dried state. However, in the case of NaCl in the growth medium, not only sucrose in the drying medium seemed to be largely ineffective in further increasing the survival rate of dried cells throughout storage, but also starvation was rather inadequate in protecting dried cells survival during storage.

Key words: lactic acid bacteria, survival, growth medium, cryoprotector, starvation