Construction of A Replicon-deficient Plasmid of Two-component Food-grade Selectable Marker for Lactococcus lactis

Abstract

Abstract: The two-component food-grade selectable marker for Lactococcus. lactis NICE system (the nisin-controlled expression system) has the advantages of dominant and complementation markers. One component is a replicon-deficificient companion plasmid, which carries an erythromycin resistance gene (Emr) as a selectable marker and it’s replication is depended on the other food-grade cloning vector which carries the functional replicon, is entirely made up of L.lactis DNA and has no selection marker. The pMG36e and pRAF800 are the sources of Emr and rep for the pEmr:rep. The rep B-deficient companion plasmid can be constructed by enzyme digestion, deficiency and ligation and coexisting with the theta replicon only.

Key words: , Lactococcus lactis； companion plasmid； two-component selection marker；

XU Bo, CAO Yu-Sheng, ZHOU Yan-Qin. Construction of A Replicon-deficient Plasmid of Two-component Food-grade Selectable Marker for Lactococcus lactis[J]. FOOD SCIENCE, 2007, 28(7): 315-319.

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Construction of A Replicon-deficient Plasmid of Two-component Food-grade Selectable Marker for Lactococcus lactis

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