Purification of Lactoferrin from Bovine Colostrum with Strong Cation Exchange Chromatography on Pilot Scale

Abstract

Abstract: A stepwise procedure for purification of lactoferrin (LF) extracted from bovine colostrum by ion exchange chromatography on a pilot scale was investigated. SP Sepharose Fast Flow (SP Sepharose FF) of excellent absorption speciality for LF, was chosen as the ion exchanger with elution rate of 2 L/h. 0.5 mol/L NaCl aqueous solution was used to elute the secretory immunoglobulin A and lactoperoxidase. Then, lactoferrin was eluted with 1.0 mol/L NaCl aqueous buffer. Lactoferrin fraction is shown as a single band in SDS-PAGE with molecular weight of 80400 Da. The isoelectric point of lactoferrin is 8.65 determined by isoelectric focusing. The purity of refined LF on pilot production is 94.20% with a yield of 75.45%.

Key words: lactoferrin, purification, strong cation exchange chromatography, pilot scale

LU Rong-Rong, XU Shi-Ying, WANG Zhang, YANG Rui-Jin. Purification of Lactoferrin from Bovine Colostrum with Strong Cation Exchange Chromatography on Pilot Scale[J]. FOOD SCIENCE, 2007, 28(7): 48-53.

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Purification of Lactoferrin from Bovine Colostrum with Strong Cation Exchange Chromatography on Pilot Scale

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