FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (8): 408-410.

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Determination of Patulin in Apple Juice Concentrated by High Performance Liquid Chromatography

 LI  Wei-Hua, DU  Li-Jun, SONG  Huan   

  1. Shanxi Entry-Exit Inspection and Quarantine Bureau, Taiyuan 030024, China
  • Online:2007-08-15 Published:2011-10-24

Abstract: A analytical method based on high performance liquid chromatography-ultravioiet (HPLC-UV) has been established for determination of patuin in apple juice concentrated. Clean-up of samples was performed with gel permeation chromatography. The calibration curve presented good linear relationship in the range of 0.01~10.0 mg/L, and the correlation coefficient was above 0.9999. Recovery for patuin at different spiked levels were range from 83%~107% with relative standard deviations of 7.8%~11.4%. Detection limit of the method for patuin was 0.005 mg/kg. The method is simple with good reproducibility and selectivity, and high sensitivity.

Key words: high performance liquid chromatography, gel permeation chromatography, concentration online, patuin, apple juice concentrated