Research on Determination of Ochratoxin A by Improved ELISA Method

Abstract

Abstract: Tiny-shake was used to shorten the detection time of enzyme linked immunosorbent assay (ELISA) for ochratoxin A (OTA). OTA from wheat was determined by ELISA. It was validated that preparing the standard solution of OTA with wheat extract solution could eliminate interference of substances in wheat. The extraction solvent was also optimized. Results showed that higher extraction rate can be achieved with NaCl-H2O-methanol (10%:70:30). The recoveries of OTA spiked at three levels, namely, 0.1, 1, 10μg/kg were 102%, and the coefficient of variation was less than 10%.

Key words: OTA, ELISA, extraction solvent, wheat

SHI Jin, HUANG 飚, SUN Wei-Rong, ZHANG Jue, ZHAO Xiao-Lian. Research on Determination of Ochratoxin A by Improved ELISA Method[J]. FOOD SCIENCE, 2007, 28(8): 425-428.

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Research on Determination of Ochratoxin A by Improved ELISA Method

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