Abstract: Tricholoma mongolicum was used as material to prepare polypeptide,and the material was processed through supercritical CO2 fluid extraction and extrusion technology before enzymatic degradation.Tricholoma mongolicum polypep-tide was prepared by two-stage enzymatic hydrolysis.The first stage use alcalase as hydrolysis enzyme to prepare polypeptide,and the second stage choose flavourzyme to adjust the flavor.The major factors that affected enzymatic degradation of Tricholoma mongolicum protein and product’s flavor were studied through orthogonal test.The optimum conditions of enzymatic hydrolysis used alcalase as hydrolysis were established:temperature 55 ℃,time 2.5 h,substrate concentration 2%,concentration of enzyme 1.5%(E/S),the first product were adjusted in flavor utilized flavourzyme,and the polysaccha-ride removed used three times volume 65% alcohol solution,yield ratio of Tricholoma mongolicum polypeptide prepared in this research is 32.10%.

Key words: Tricholoma mongolicum, polypeptide, supercritical CO2 fluid extraction, extrusion technology, enzymatic hydrolysis