FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (9): 274-277.

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Extraction of Polysaccharide in Lentinus edodes by Enzymatic Method

 LI  Bo, SONG  Jiang-Liang, ZHAO  Sen, ZHOU  Chong, LIU  Qian-Qian   

  1. School of Food Science,Henan Institute of Science and Technology,Xinxiang 453003,China
  • Online:2007-09-15 Published:2011-11-22

Abstract: Papain and cellulase were applied on the extraction of lentinan,and the extraction conditions of enzymatic method were studied in this research.The results showed that the optimal conditions of papain were enzymatic concentration 0.5%,temperature 50 ℃,pH6~7 and reaction time 1 h.The optimal conditions of cellulase were enzymatic concentration 0.25%,temperature 40 ℃,pH4.5~5.0 and reaction time 1 h.By enzymatic hydrolysis,the extraction yield of lentinan was improved distinctly.

Key words: lentinan, extraction, papain, cellulase