FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (9): 353-356.

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Effects of Different Culture Methods on Genomic DNA Extraction from Pleurtus citrinopileatus

 SU  Hong-Yan, WANG  Lei, WANG  Yan-Jie, GUO  Wen-Wen   

  1. College of Life Sciences,Ludong University,Yantai 264025,China
  • Online:2007-09-15 Published:2011-11-22

Abstract: The mycelium of Pleurtus citrinopileatus 515 was cultured by five different culture methods.The genomic DNA was then extracted by improved SDS method and the quality was evaluated by agarose electrophoresis and absorbency analysis.The results indicated that the mycelium cultured by static liquid culture and shaking-bottle liquid culture grew well,and genomic DNA extraction efficaiency was higher than other culture methods.However,the DNA from mycelia cultured by static liquid cultivation contained plenty of impurity such as protein or amylose.Therefore the shaking-bottle liquid culture is the best culture method to extract genomic DNA from Pleurotus citrimopileatus.

Key words: Pleurotus citrnopileatus, culture method, genomic DNA, extraction