Mutagenesis Breeding of Strain for Chitinase Production and Optimization of Fermentation Conditions in Shaking Flasks

Abstract

Abstract: A higher chitinase-producing mutant with stable heritage,TUC13,was obtained from initial strain TS30,which was separated from soil at earlier research stage,by means of UV+LiCl mutagenization.The optimum medium and fermentation conditions for TUC13 have been established through orthogonal test design.Under the established optimal culture conditions and in the established optimal medium,the chitinase activity in broth of TUC13 increased by 236.5% compared with that in the broth of initial strain under the culture conditions before optimization.

Key words: chitinase, mutagenesis, fermentation condition

TIAN Qiang, ZHANG Cai, WU Zi-Jian. Mutagenesis Breeding of Strain for Chitinase Production and Optimization of Fermentation Conditions in Shaking Flasks[J]. FOOD SCIENCE, 2007, 28(9): 405-409.

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Mutagenesis Breeding of Strain for Chitinase Production and Optimization of Fermentation Conditions in Shaking Flasks

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