FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (10): 267-269.

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Study on Extraction Mothod of Phaseolus vulgaris Agglutinin

 LI  Xiao-Mei   

  1. College of Food Engineering,Harbin University of Commerce,Harbin 150076,China
  • Online:2008-10-15 Published:2011-12-08

Abstract: The factors such as extraction agent,liquid ratio,ultrasonic time,and ultrasonic power affecting on the agglutinin extraction from Phaseolus vulgaris were investigated. The effects of hemagglutination culture time and the centrifugal speed on hemagglutination effect and dry weight were also discussed. The results showed that saline as the extraction agent,the optimal extraction conditions determined by orthogonal test are: material-liquid ratio 1: 10,ultrasonic time 30 min,and ultrasonic power 350 W. The hemagglutination culture time and centrifugal speed are 3 h and 3000 r/min,respectively.

Key words: Phaseolus vulgaris agglutinin, extraction, hemagglutination effect, dry weight