Study on Isolation,Purification and Preliminary Structural Analysis of Immunological Glycoprotein from Corbicula fluminea

Abstract

Abstract: The glycoprotein from Corbicula fluminea was isolated and purified by the methods of EDAE-52 cellulose and Sephadex G-75 column chromatography.The purity of the glycoprotein is 94.74% and its molecular weight is19030D by RP-HPLC and SDS-PAGE analysis.It has 5.18% sugar by phenol-sulfuric acid reaction and 93.22% protein by Bradford method.Alkaline β-elimination reaction proved protein is covalently linked with carbohydrate by O-glycosyl linkage.The glycoprotein by IR analysis shows the typical absorption of polysaccharide and the presence of pyranose β-glycoside linkage is indicated.

Key words: glycoprotein from Corbicula fluminea, isolation and purification, configuration analysis

XU Ming-Sheng, ZENG Ting-Ting, JIANG Yan, DU Hua-Ying. Study on Isolation,Purification and Preliminary Structural Analysis of Immunological Glycoprotein from Corbicula fluminea[J]. FOOD SCIENCE, 2008, 29(11): 157-160.

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Study on Isolation,Purification and Preliminary Structural Analysis of Immunological Glycoprotein from Corbicula fluminea

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