FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (11): 298-299.

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Study on Separation and Purification of Chlorogenic Acid with High-speed Countercurrent Chromatography

 CHEN  De-Jing, HAN  Hao, DONG  Bo-Ni   

  1. 1.Shaanxi University of Technology,Shanxi Provincial Bio-resource Key Laboratory,Hanzhong 723000,China;2.College of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723000,China
  • Online:2008-11-15 Published:2011-12-08

Abstract: High-speed countercurrent chromatography was used to separate chlorogenic acid from 70% ethanol extract of dry Lonicera japonica Thunb.with three different solvent systems.It was found experimentally that the optimal solvent system is composed of butanol,acetic acid and wate,with the ratio of 4:1:5(V/V).The purity of obtained chlorogenic acid is determined as 98.1% by high performance liquid chromatograph(HPLC),and its yield is 95%.

Key words: chlorogenic acid, high-speed countercurrent chromatography(HSCCC), seperation