Isolation of Phaseolus vulgaris Agglutinin and Determination of Its Molecular Weight

Abstract

Abstract: The effects of(NH4)2SO4 saturation and pH on the isolation of Phaseolus vulgaris Agglutinin(PVA) were investigated in this study.PVA content and molecular weight were determined by using agglutinin biological activity and SDS-PAGE electrophoresis methods respectively.The optimal isolation conditions are as follows:(NH4)2SO4 saturation 60 % and pH 5.5.The content of agglutinin is 189.7 mg/100 g under such conditions.The optimum conditions of SDS-PAGE electrophoresis are as follows:Sample concentration 10 mg/g,injection volume 20 μl,separating gel concentration 12%,voltage 50V and current 25 mA.The relative molecular weight is 204.07 kD under the above conditions.

Key words: Phaseolus vulgaris agglutinin(PVA), isolation, content, molecular weight

LI Xiao-Mei. Isolation of Phaseolus vulgaris Agglutinin and Determination of Its Molecular Weight[J]. FOOD SCIENCE, 2008, 29(11): 328-331.

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Isolation of Phaseolus vulgaris Agglutinin and Determination of Its Molecular Weight

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