Abstract: Oviductus Ranae of Rana temporaria chensinensis David was hydrolyzed by the combination of caroid and alkaline proteinase,and the gel chromatography and HPLC were employed for determination.The result showed that the optimal hydrolysis conditions by caroid are:substrate concentration 1%,enzyme dosage 1200 U/g,temperature 60 ℃,time 3.5 h and pH 6.0.By alkaline proteinase they are enzyme dosage 500 U/g,temperature 50 ℃,time 3.0 h,and pH 8.0.Molecular weight less than 1355 D peptid was obtained by Sephadex G-15 separating column,and three peaks appearanced in the chromatogram by HPLC detection,whose retention time was 21.837,23.300 and 24.499 min in turn.Meanwhile,the Oviductus Ranae protein can be effectively degradated into peptide by the combination of the two enzymes,and the degree of hydrolysis reaches 17.8%,increasing by 5.6% compared with single enzyme.The production dissolubility and flowability are both better.

Key words: Oviductus Ranae, enzymolysis, micromolecule peptide