Study on Properties of Myrosinase from Radish

Abstract

Abstract: Using glucosinolates from radish seed extract as substrate,the properties of myrosinase from radish were investigated.Results indicated that the optimum temperature of myrosinase is 50℃,and the optimum pH values are 4.0 and 8.0.Ascorbic acid can activate the enzyme,whose activity reaches a climax of 107 U at the ascorbic acid concentration of 5 mmol/L.L-Cysteine hydrochloride and glutathione also increase the myrosinase activity.However,NaCl,Na2SO3 and citric acid have inhibitory effects on the enzyme activity.Fe2+,Mg2+,Al3+ and Ca2+ have different effects on the myrosinase with different kinds of metal ions.

Key words: radish, myrosinase, enzymatic properties

LI Jie, WANG Qing-Zhang, ZHOU Qi, SUN Yin, XIE Bi-Jun. Study on Properties of Myrosinase from Radish[J]. FOOD SCIENCE, 2008, 29(11): 451-455.

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Study on Properties of Myrosinase from Radish

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