Abstract: The kinetics of a chitinase from TUV26 strain,a high chitinase-producing strain which was obtained from a strain of Pseudomonas sp.through mutation breeding,was studied in detail.Results showed that toward colloidal chitin,the optimum conditions of enzymic reation by chitinase are 48.5 ℃ and pH 6.8,and under those optimum conditions the reaction progress curve 0 to 60 minutes is in the straight line stage and the initial velocity is 54.199 μg/ml·h.Km,the Michaelis constant of chitinase,is 24.04 μg/ml,Vmax,the maximum reactive velocity,is 218.68 μg/ml·h,and Kcat,the enzymatic turnover number,is 0.08025 h-1.These results provide technical parameters for the production of chitooligosaccharides by chitinase.

Key words: chitinase, enzyme kinetics, Michaelis constant