Purification and Characterization of Chitinase Inhibitor from the Fermentation Products of Actinomycete A0101

doi:10.7506/spkx1002-6630-201519035

Abstract

Abstract:

Bombyx mori chitinase was used as the target to screen an actinomycete strain able to inhibit chitinase by
transparent ring plate screening and DNS colorimetric screening methods. The strain A0101 with high inhibitory specificity
was obtained. The activity-guided separation and purification of the fermented products of strain A0101 were carried out
by alcohol precipitation, dialysis, silica gel column chromatography and Sephadex G-15 gel column chromatography, and
a pure bioactive material was obtained. This bioactive material exhibited a single HPLC peak with a retention time of 4.1 min. Its
inhibitory rate on chitinase was evaluated by DNS colorimetric method to be 80.2%. Moreover, it proved to be a multiple hydroxyl
carbohydrate according to the infrared (IR), 1H-nuclear magnetic resonance (NMR) and mass spectrometry analyses.

Key words: chitinase, inhibitor, purification, characteristics

CLC Number:

TS201.3

GU Donghua, ZHANG Hongbin*, LI Qiuping, LI Yao, HU Xueqin. Purification and Characterization of Chitinase Inhibitor from the Fermentation Products of Actinomycete A0101[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201519035.

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Purification and Characterization of Chitinase Inhibitor from the Fermentation Products of Actinomycete A0101

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