FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (12): 259-263.

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Study on Optimization of Inclusion Process of Paeonol with Bletilla striata(Thunb.) Reichb. f. Polysaccharides(BSPS) by Orthogonal Design and Identification of Inclusion Complex

 WANG  Ru-Ping, WU  Ting, ZHANG  Wei-Ming, WU  Guo-Rong   

  1. 1.Yangzhou Vocational College of Environment and Resources,Yangzhou 225007,China;2. Jiangsu Key Laboratory of Supramolecular Medicinal Materials and Applications,Nanjing 210046,China;3. Nanjing Institute for Comprehensive Utilization of Wild Plant,Nanjing 210042,China
  • Online:2008-12-15 Published:2011-12-08

Abstract: Orthogonal test design L9(34) was used to optimize the preparation process of inclusion compound of paeonol-BSPS in this study,and differential thermal analysis meter(DTA) ,IR-spectrum and nuclear magnetic resonance(NMR) . were used to identify the inclusion. Results showed that the optimal preparation process of inclusion are as follows:ratio of paeonol-BSPS 1:6,temperature 30 ℃,water bath time 4 h,and by this process the inclusion rate and yield are 29.38% and 74.29%,respectively. The results of DTA,IR-spectrum and NMR showed that the inclusion has formed. The mechanism of inclusion of paeonol with BSPS was discussed.

Key words: paeonol, Bletilla striata(Thunb.) Reichb. f., polysaccharide(BSPS), orthogonal design, inclusion, identification