Optimization of Glucosinolates Extraction Process from Radish Seeds by Orthogonal Rotation Design

Abstract

Abstract: On the foundation of the single-factor tests,the quadratic orthogonal rotation design test was carried out with three factors including extraction temperature,water-material ratio,extraction time to study glucosinolates extraction from radish seeds. A quadratic regress equation on glucosinolates yield was established,and the theoretical model was fitted well. Through response surface analysis and simulation analysis,the optimum combination conditions were obtained. Results indicated that extraction temperature has a remarkable influence on glucosinolates extraction rate,but water-material ratio and extraction time have no remarkable influence. When the extraction temperature ranges from 4.3 ℃ to 16.5 ℃,the extraction time ranges from 20 min to 57 min,and the solid-liquid ratio ranges from 9.4 to14.2 ml/g,the concentraion of glucosinolates in radish sead extract is above 0.9 mg/ml.

Key words: radish seeds, glucosinolates, extraction, orthogonal rotation design

LI Jie, TAN Zheng-Lin, WANG Qing-Zhang, ZHANG Jin-Qiu, CHEN Xing, XIE Bi-Jun. Optimization of Glucosinolates Extraction Process from Radish Seeds by Orthogonal Rotation Design[J]. FOOD SCIENCE, 2008, 29(12): 264-269.

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Optimization of Glucosinolates Extraction Process from Radish Seeds by Orthogonal Rotation Design

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