Cloning and Expression of Thermus aquaticus FL-03 Thermophil Amylomaltase Gene

Abstract

Abstract: MalQ gene was amplified by PCR with Thermus aquaticus FL-03 genome DNA,the amplified MalQ gene was cloned into prokaryotic expression vector pET-28a(+) and expressed in BL21(DE3) under induction of IPTG. The expressed product was identified with SDS-PAGE and Western blotting. The amylomaltase was purified by inactivation at 70 ℃ and centrifugalization. The glucanothansferase activity of the purified amylomaltase was identified via TLC method with 10% maltose as substrate. The specific activity is improved 5 folds and the residual activity is 70% after purification. The amylomaltase has great potential in application of various industries.

Key words: Thermus aquaticus FL-03, amylomaltase, genecolone prokaryotic expression, purification

LIU Jia-Huan, YU Han-Song, WANG Yu-Hua, HU Yao-Hui. Cloning and Expression of Thermus aquaticus FL-03 Thermophil Amylomaltase Gene[J]. FOOD SCIENCE, 2008, 29(12): 389-392.

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Cloning and Expression of Thermus aquaticus FL-03 Thermophil Amylomaltase Gene

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