Optimization of Enzymatic Preparation Conditions of Functional Polypeptide from Male Silkworm Moth Protein

FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (12): 417-421.

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Optimization of Enzymatic Preparation Conditions of Functional Polypeptide from Male Silkworm Moth Protein

ZHANG Lu, TANG Dao-Bang, XIAO Geng-Sheng, LIU Ji-Ping, XU Yu-Juan, ZOU Yu-Xiao

1. Sericulture and Farm Product Processing Research Institute,Guangdong Academy of Agricultural Science,Guangdong Open Access Laboratory of Agricultural Product Processing,Guangzhou 510610,China;2. School of Animal Science,South China Agricultural University,Guangzhou 510642,China

Online:2008-12-15 Published:2011-12-08

Abstract

Abstract: Taking hydrolysis degree and peptide yield as an indexes,the effect of temperature,pH,substrate concentration,alkaline amount on enzymolysis of male silkworm moth protein were studied. The optimal conditions are determined by orthogonal rotation combination design as follows:64.5 ℃,pH 9.36,substrate concentration 4.46%,protease amount 6660 U/ml;and reaction time 300 min. Under these conditions,the hydrolysis degree is up to 21.65% and the peptide yield is up to 34.81%.

Key words: male silkworm moth, functional polypeptides, alkaline protease, orthogonal rotation combination design

ZHANG Lu, TANG Dao-Bang, XIAO Geng-Sheng, LIU Ji-Ping, XU Yu-Juan, ZOU Yu-Xiao. Optimization of Enzymatic Preparation Conditions of Functional Polypeptide from Male Silkworm Moth Protein[J]. FOOD SCIENCE, 2008, 29(12): 417-421.

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Optimization of Enzymatic Preparation Conditions of Functional Polypeptide from Male Silkworm Moth Protein

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