Abstract: Vibrio parahaemololyticus is the main cause of foodborne disease,and it is also a common pathogen detected from seafood. Vibrio parashaemoloyticus would cause enormous loss when fish and shrimp and so on are infected by it. In this study,laying hens were divided into two groups,and were immunized with two different doses of prepared antigen. The low dose group of hens was immunized with 5×107 CFU/ml,and the high dose group of hens was immunized with 5×108 CFU/ml Vibrio parahaemololyticus. ELISA was established to detect the antibody activity. The results demonstrated that the highest titer of IgY against Vibrio parahaemololyticus in the high and low dose group could reach 1:57600 and 1:28800,respectively. The titer of IgY produced by the low dose group could maintain for 250 days,but the titer of IgY produced by the high dose group could maintain for more than 300 days.

Key words: Vibrio parahaemololyticus, IgY, production, antibody activity