Determination of Lincomycin Residues in Milk with HPLC

Abstract

Abstract: A method was developed for detecting lincomycin residues in milk with HPLC with DAD detection at 210 nm. Lincomycin was extracted from milk with water and centrifuged. The solution was cleaned using C18 solid-phase extraction cartridge,and Agilent ZORBAX SB C18(4.6 mm×250 mm,5 μm) column was used. The mobile phase consisted of borax solution(adjusting pH to 6.0 with phosphoric acid ) and methanol( 55:45,V/V ) and the flow rate was 1.0 ml/min. The results showed that the peak area response has linear relationship with lincomycin concentration between 1.0 and 10.0 μg/g. The average recovery rate is 82.9% to 93.1 % and the coefficient of variation is 1.45% to 4.58%. The detection limit for lincomycin is 0.5 μg/ml. The method is simple,rapid and precise.

Key words: high-performance liquid chromatography(HPLC), lincomycin, milk

CHEN Ming, GENG Zhi-Ming. Determination of Lincomycin Residues in Milk with HPLC[J]. FOOD SCIENCE, 2008, 29(12): 572-574.

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Determination of Lincomycin Residues in Milk with HPLC

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