Abstract: It is a hot research topic in enzyme engineering that bioactive peptides substance releasing from animal and plant protein by enzymatic methods. Immobilized enzyme technique is one of the core technologies of enzyme engineering, which is widely applied in the life science, bio-medicine products, food science, chemical and environmental science, and so on. The focus of immobilized enzyme technique is to develop new carrier materials and design simple, convenient, extremely efficient, mild conditions of the immobilized method. Based on our research, this paper mainly summarized the carrier materials such as chitosan and nanostructures, and technologies of immobilized enzyme such as oriented immobilization, and research advance in immobilization of protease and its development of bioactive components.

Key words: protease, chitosan, nanostructures, oriented immobilization, bioactive components