Screening and Optimization on Medium of Bifidobacterium

Abstract

Abstract: Base medium of Bifidobacterium was screened for maximum cell mass among 4 kinds of media, which was the best to obtain maximum cell counts for Bifidobacterium longum. By orthogonal design, on the base medium the optimal growth medium was obtained through changing carbon source, adding fructo-oligosaccharid and adjustment endogenesis of pH. In optimal growth medium the viable cell counts of B.longum is about 8.1 times higher than the base medium and the culture time shortens 4 h. The viable cell counts of B.longum can reach 8.9×109 CFU/ml in the optimal growth medium at 37 ℃ for 20 hours under anaerobic condition.

Key words: Bifdobactrium, media, screening, optimization

SHAO Jian-Ning, GONG Wei-Zhong, MA He-Ping, PENG Zhang-Pu. Screening and Optimization on Medium of Bifidobacterium[J]. FOOD SCIENCE, 2008, 29(3): 318-321.

[1]	WU Bohua, JIANG Xuewei, ZHANG Jinyu, RUAN Zhiqiang, LIANG Shengnan, CHEN Yongfa, ZHOU Shangting. Screening and Application of Bacillus spp. Promoting Moromi Fermentation [J]. FOOD SCIENCE, 2021, 42(6): 134-141.
[2]	ZHU Xiaoling, LIU Jie, YU Tingting, ZHANG Li, WANG Huixia, WU Wanqin, CAO Qi, ZHANG Yazhen, YANG Zong. Rapid Screening of Pesticide Residues in Pepper Powder by High Performance Liquid Chromatography Coupled with Quadrupole-Time of Flight Mass Spectrometry [J]. FOOD SCIENCE, 2021, 42(4): 287-296.
[3]	CHEN Long, YANG Jun, MA Maomao, WU Shasha, YU Ping, ZENG Zheling. Strain Screening and Optimization of Fermentation Conditions for Protease Production Using Rapeseed Meal as a Nitrogen Source [J]. FOOD SCIENCE, 2021, 42(4): 115-121.
[4]	SUN Yue, LIU Jiayi, CHEN Lu, DU Hong, BAI Fengling, LÜ Xinran, ZHANG Defu, GUO Xiaohua, LI Jianrong. Screening of Lactic Acid Bacteria for Antagonistic Activity against Drug-Resistant Escherichia coli and Underlying Mechanism [J]. FOOD SCIENCE, 2021, 42(2): 121-127.
[5]	WANG Chan, LI Rong, WU Yang, WANG Ying, TANG Shuhua, TAN Jin, JIANG Zitao. Rapid Screening of Radical Scavengers in Essential Oil from Piper longum L. as Food Seasoning [J]. FOOD SCIENCE, 2021, 42(14): 226-231.
[6]	LI Yijuan, SHAO Kaisheng, ZHANG Na, MENG Yingping, TAO Xueying, ZHOU Yingyu, WEI Hua, ZHANG Zhihong. Directional Screening for Reuterin-Producing Lactobacillus reuteri and Its Biological Characteristics [J]. FOOD SCIENCE, 2021, 42(14): 32-38.
[7]	CHEN Zhenjin, ZHANG Huang, SHI Lei, YE Lei, CAI Kaixian, YAN Donglin, WEI Tao, XIE Naizhen, CHEN Xiaocong. Rapid Detection of Murine-Derived Ingredients in Mutton Products Using Loop-Mediated Isothermal Amplification [J]. FOOD SCIENCE, 2021, 42(12): 322-327.
[8]	PAN Fei, ZHAO Lei, CHEN Yanlin, HAO Shuai, ZHANG Huimin. Preparation, Characterization and Sustained Release Property of Black Rice Anthocyanin-Loaded Chitsan γ-Polyglutamic Acid Nanoparticles [J]. FOOD SCIENCE, 2021, 42(10): 38-44.
[9]	DING Lili, LÜ Xinran, GAO Yongyue, CUI Xiaoling, WANG Xiaomi, BAI Fengling, YI Shumin, GUO Xiaohua. Screening for and Identification of Lactic Acid Bacteria with Antioxidant Activity from the Intestinal Tract of Fish [J]. FOOD SCIENCE, 2021, 42(10): 127-132.
[10]	ZHOU Zhiqiang, MA Jinju, GAN Jin, LI Kun, LI Kai, ZHANG Wenwen, TU Xinghao, DU Liqing, ZHANG Hong. Effect of Bleached Shellac/Tannic Acid Composite Coating on Mango Preservation at Room Temperature [J]. FOOD SCIENCE, 2020, 41(9): 145-152.
[11]	ZHANG Yinghao, XUE Zhaoyang, ZHAO Tingbin, YIN Haisong, QIAO Changsheng,. Construction and Application of a Predictive Model for Determination of Polymalic Acid in the Fermentation Broth of Aureobasidium pullulans by Near-Infrared Spectroscopy [J]. FOOD SCIENCE, 2020, 41(8): 152-158.
[12]	SU Jiajia, YANG Tian, TONG Enjie, ZHAO Xueying, HE Chenghao, YANG Na, XU Xueming, WU Fengfeng. Optimization of Brewing Process for Sweet Rice Wine from Brow Rice and Analysis of Its Volatile Components [J]. FOOD SCIENCE, 2020, 41(8): 177-185.
[13]	LIU Shuxin, WU Aijuan, ZHEN Ni, SUN Jie, HUANG Ling, ZENG Zhidan, ZENG Xiaoqun, PAN Daodong. Screening for a Strain of Lactic Acid Bacteria with Broad-Spectrum Antimicrobial Activity and Analysis of Its Bacteriocin-Related Genes [J]. FOOD SCIENCE, 2020, 41(6): 101-107.
[14]	ZHANG Shixin, PENG Litao, FAN Ming, YANG Shuzhen, YAN Deng. Screening for Antifungal Agents of Clinical Drugs against Penicillium expansum on Postharvest Pear Fruit [J]. FOOD SCIENCE, 2020, 41(5): 180-185.
[15]	SONG Yifan, CHEN Haifeng, YUAN Yuejin. Optimization of CO2-Low Temperature High Pressure Permeation Drying Process of Kiwifruit [J]. FOOD SCIENCE, 2020, 41(4): 229-234.

Screening and Optimization on Medium of Bifidobacterium

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments