Screening and Optimization on Medium of Bifidobacterium

Abstract

Abstract: Base medium of Bifidobacterium was screened for maximum cell mass among 4 kinds of media, which was the best to obtain maximum cell counts for Bifidobacterium longum. By orthogonal design, on the base medium the optimal growth medium was obtained through changing carbon source, adding fructo-oligosaccharid and adjustment endogenesis of pH. In optimal growth medium the viable cell counts of B.longum is about 8.1 times higher than the base medium and the culture time shortens 4 h. The viable cell counts of B.longum can reach 8.9×109 CFU/ml in the optimal growth medium at 37 ℃ for 20 hours under anaerobic condition.

Key words: Bifdobactrium, media, screening, optimization

SHAO Jian-Ning, GONG Wei-Zhong, MA He-Ping, PENG Zhang-Pu. Screening and Optimization on Medium of Bifidobacterium[J]. FOOD SCIENCE, 2008, 29(3): 318-321 https://doi.org/

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Screening and Optimization on Medium of Bifidobacterium

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