Analyzing Conservativeness of pksCT Gene for Citrinin Biosynthesis from Citrinin-producing Monascus spp

Abstract

Abstract: pksCT gene was a related gene responsible for citrinin biosynthesis in Monascus purpureus. In this study, fourteen citrinin-producing Monascus spp. coming from seven Monascus species were used in the experiment. Two DNA fragments from the transcriptional start region and the stop codon region of pksCT gene, which were 669 bp and 591 bp respectively, were gained from fourteen Monascus spp. by genomic PCR. Results of NCBI BLAST showed that PCR products exhibited high identity with each other, and were in accordance with the partial sequences of pksCT gene in M.purpureus which was announced in the Genbank. It was suggested that pksCT gene was a general gene responsible for citrinin biosynthesis in Monascus species. It was also indicated that it was the effective way to distinguish the citrinin-producing Monascus strain by analyzing the pksCT gene with genomic PCR.

Key words: Monascus, citrinin biosynthesis gene, conservativeness

FU Gui-Ming, XU Yang, LI Yan-Ping, WU Chou-Fei. Analyzing Conservativeness of pksCT Gene for Citrinin Biosynthesis from Citrinin-producing Monascus spp[J]. FOOD SCIENCE, 2008, 29(3): 359-363.

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Analyzing Conservativeness of pksCT Gene for Citrinin Biosynthesis from Citrinin-producing Monascus spp

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