FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (3): 374-379.

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Analysis of Organic Acids in Fermented Capsicum by Reverse Phase High Performance Liquid Chromatography

JIA  Hong-Feng, HE  Zhi-Fei, LI  Hong-Jun, FANG  Ming-Qiang, LANG  Jia, ZHU  Yan-Fang   

  1. 1.Depertment of Food Science, Sichuan Higher Institute of Cuisine, Chengdu 610072, China; 2.College of Food Science, Southwest University, Chongqing 400716, China; 3.Chongqing Fuling Zhacai Group, Chongqing 408000, China
  • Online:2008-03-15 Published:2011-08-24

Abstract: A method for simultaneous determination of 8 organic acids in fermented Capsicum by reverse phase high performance liquid chromatography was developed. Hypersil-ODS2 column(4.6 mm×200 mm, 5μm)was used at 17℃ and mobile phase was 2% CH3OH-0.05 mol/L KH2PO4(pH 2.8)with flow rate of 0.8 ml/min. Detection wavelength was 214 nm. The relative standard deviations were lower than 3%, and the recoveries were 92.87% to 106.15%. In this study, two methods (extracted with heating or ultrasonic) were used to extract organic acids, it is proved that extraction with heating is better than ultrasonic.

Key words: HPLC, fermented Capsicum, organic acids, extraction