Simultaneous Kinetic Spectrophotometric Determination of Tyrosine and Tryptophane with Aid of Chemometrics

Abstract

Abstract: A kinetic spectrophotometric method for simultaneous determination of tyrosine and tryptophane with chemometrics was developed. This method was based on the reduction of KMnO4 to MnO42-by these two amino acids in alkalescent medium. By detecting the absorbance of MnO42-at 610 nm wavelength for a period of 300 s, the kinetic data were measured and recorded for determination. The partial least square method was used to establish the model based on the kinetic data and was then used to predict the merchant amino acid samples with satisfy results.

Key words: chemometrics, kinetic spectrophotometry, tyrosine, tryptophane

NI Yong-Nian, CAI Ying-Jun. Simultaneous Kinetic Spectrophotometric Determination of Tyrosine and Tryptophane with Aid of Chemometrics[J]. FOOD SCIENCE, 2008, 29(3): 433-435.

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Simultaneous Kinetic Spectrophotometric Determination of Tyrosine and Tryptophane with Aid of Chemometrics

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