FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (4): 189-192.

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Purification and Isolation of Soybean Genistin and Daidzin

 SUN  Yan-Mei, ZHANG  Yong-Zhong, XU  Bao-Rong, YANG  Lin, LI  Mu-Zi, CHI  Yu-Jie   

  1. 1.Department of Applied Chemistry, Northeast Agricultural University; 2.Key Laboratory of Soybean Biology, Ministry of Education; 3.Harbin Third Pharmaceutical Manufactory; 4.College of Food, Northeast Agricultural University
  • Online:2008-04-15 Published:2011-08-24

Abstract: In order to study the properties of respetive monomer of genistin and daidzin as main compounds of isoflavone extracts from soybean, their respective isolation was studied in the paper. First, in order to acquire high Purity of products, study purified isoflavone extracts by acetone extraction with anhydrous alcohol recrystallization. Then, genistin and daidzin were respectively separated by Sephadex LH-20 column.

Key words: isoflavones, genistin, daidzin, purification, isolation, Sephadex LH-20