Effects of DEAE-52 Anion-exchanging Chromatography on Yield Rate of IgG Purified from Swine Serum

Abstract

Abstract: Objectives: To purify immunoglobulin G (IgG) from swine serum and analyze the effects of DEAE-52 chromatog- raphy on the yield rate of IgG. Methods: Porcine IgG was separated and purified from swine serum by two-step methods, SDS- PAGE and Bradfold assaying kit with saturated ammonium sulphate (SAS) precipitation followed by DEAE-52 cellulose anion- exchanging chromatography. The effects of DEAE-52 chromatography on the yield rate of IgG were defermined by SDS-PAGE and Bradford assaying kit with double immunodiffusion(DI). Results:Two different eluate peaks are obtained in DEAE-52 chromatography. IgG is also found in different eluate peaks of chromatogram by SDS-PAGE and DI.The yield rate and purity of IgG in first eluate peak are 3.0～4.0 mg/ml serum and 95.7% respectively. But the purity in second eluate peak is lower. Conclusion: DEAE-52 chromatography leads the porcine IgG to distribute in two different eluate perks. The IgG of electro- phoretic purity can be purified from the first eluate perk of DEAE-52 chromatogram by two-step methods. The yield rate of IgG can be largely increased by IgG and further purified from the second eluate perk .

Key words: porcine immunoglobulin G(IgG), purify, chromatography, yield, effect

LIU Sheng-Jie, YU Wei-Yi, ZHU Mao-Ying, LIU Cheng-Wen, NIE Chuan-Peng, LI Dong-Wei. Effects of DEAE-52 Anion-exchanging Chromatography on Yield Rate of IgG Purified from Swine Serum[J]. FOOD SCIENCE, 2008, 29(4): 87-89.

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Effects of DEAE-52 Anion-exchanging Chromatography on Yield Rate of IgG Purified from Swine Serum

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