Abstract: A method for the determination of residual carbendazim in vegetable was established by reverse phase high-performance liquid chromatography with DAD detector.The carbendazim was extracted with methanol and partitioned into methylene chloride after initial acidification and again after subsequent alkalinization and last acidification of the extract.The volume is adjusted to 10.0 ml with HCl(1+11);A RP-HPLC method was used with C8 column(5μm,250mm×4.6 mm).The flow rate was 1.0 ml/min and the detection wavelengh at 286 nm.The proposed method is accurate,precise and selective with limit of determination of 0.02 mg/kg,recovery of more than 70% and RSD of less than 10%.

Key words: onion, garlic, carbendazim, residues, RP-HPLC