FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (6): 139-141.

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Study on Extraction Technics and Content Variety Trends of Total Flavonoids in Lithocarpus polystachyus Rehd.

 LI  Sheng-Hua, WU  Xian-Jin, YU  Jian-Ping, LI  Si-Yuan, HAN  Yong-Guang   

  1. 1.Department of Bioengineering, Huaihua College; 2.Institution of Biochemistry and Nutrition, Guizhou University
  • Online:2008-06-15 Published:2011-08-26

Abstract: Object: To study extraction technics and variety trends of the total flavonoids in Lithocarpus polystachyus Rehd. Method: Using design of L9 (34), the extraction conditions of total flavonoids from Lithocarpus polystachyus Rehd. in Huaihua were optimized by ethanol solution extraction assisted with ultrasonic , and the variety trends of total flavonoids content were investigated with different natural storage time, different gathering seasons, differrent Lithocarpus polystachyus Rehd. leaves. Result: Extraction temperature had effect on total flavonoids yield, and ethanol concentration had more obvious effect than extraction temperature. The more natural storage time, the less total flavonoids content, and the average descent rate was 24.74% per year. The flavonoids content from Lithocarpus polystachyus Rehd. gathered between August and October was the highest. The total flavonoids content was higher in the new leaves of Lithocarpus polystachyus Rehd.in Huaihua than in the old leaves, and the content was the least in stem. Conclusion: The optimum conditions for extracting total flavonoids from Lithocarpus polystachyus Rehd. were material to 70% ethanol concentration ratio 1:35, ultrasonic power 540 W, and extraction time15 min.

Key words: Lithocarpus polystachyus Rehd., total flavonoids, extraction technics, content, variety trends