Abstract: Salmonella, Shigella and Escherichia coli O157:H7 are important food-borne pathogens in meat and its products, therefore it is very useful to establish rapid detection method for controlling the quality of meat and its products. Three sets of specific primers were designed according to the gene segment of invA of Salmonella, ipaH of Shigella and rfbE of Escherichia coli O157:H7, and then the reaction parameters were optimized to develop a PCR detection kit. The results showed that the multiplex PCR method is simple and rapid, the sensitivity achieved is as low as 1 CFU/g and the whole detection time is less than 24 hours. The capacity of the detection kit is rapid and accurate, so it could be wildly used in many fields such as food sanitation detection, clinical inspection and so on.

Key words: meat product, food-borne pathogenic microorganism, multiplex PCR, detection kit