FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (7): 133-137.

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Breaking of Waste Brewer Yeast with Pulsed Electric Fields Combined with Ultrasound Treatment

 XIE  Ge, YANG  Rui-Jin, LU  Rong-Rong, ZHANG  Wen-Bin, ZHAO  Wei   

  1. 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 2. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
  • Online:2008-07-15 Published:2011-07-28

Abstract: Combined treatment with pulsed electric fields (PEF) and ultrasound was used to extract protein and nucleic acid from waste brewer yeast cell. Experiment was carried out with PEF (667 pps at 25 kV/cm, 1584μs) treatment followed by ultrasound (400 W, 15 min) treatment. The results showed that the highest extraction rate of protein and nucleic acid reaches 45.86% and 53.75%, respectively, almost 1.5 times of extraction rate of protein and nucleic acid only by PEF, and 2 times only by ultrasound.

Key words: waste brewer yeast, pulsed electric fields (PEF), ultrasound, protein, nucleic acid