Abstract: The process parameters for preparing liposomal propolis flavone extract were preliminarily optimized using uniform design with mean diameter of liposome,encapsulation efficiency,and storage stability of liposome as major indexes. Based on in vitro controlled release characteristics of liposomal propolis flavone extract,its optimum preparation conditions are determined as follows:egg lecithin 500 mg,cholesterol 80 mg,propolis 100 mg,rotary evaporating temperature 45 ℃,phosphate buffer solution(pH 7.4) 25 ml and ultrasonic treatment time 25 min. The resultant liposomal propolis flavone extract shows an encapsulation efficiency of 86.6% with a mean diameter of 104.78 nm and a percentage encapsulation efficiency retention rate of 77.0%.

Key words: propolis flavone extract, liposome, uniform design, in vitro release characteristics