FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (8): 418-421.

Previous Articles     Next Articles

Effects of Benzo[a]pyrene on Expression of XPA and XPC Genes of A549 Cells

 GONG  Zhi-Yong, JIANG  Qi, WANG  Yao-Feng, FANG  Min   

  1. 1.School of Food Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China;2.Key Laboratoyg of Environment and Health,Ministry of Education,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
  • Online:2008-08-15 Published:2011-08-26

RichHTML

0

PDF (PC)

342

Abstract: Objective:To investigate the effects of benzo [a] pyrene(BaP) on expression of XPA and XPC genes involved in DNA repair of A549 cells. Methods:A549 cells were exposed to different concentrations of benzo[a]pyrene(1,5,10,50 and 100μmol/L BaP) for 24 h. Cell survival was measured using MTT assay,and mRNA expression levels of XPA and XPC genes of the cells were detected using real-time quantitative RT-PCR. Results:A549 cells growth rates significantly decrease growth rate compared with the group not exposed to BaP. And the mRNA expression levels of XPA and XPC genes significanty decreased compared with the group not exposed to BaP. Conclusion:Benzo[a]pyrene could repress mRNA expressions of XPA and XPC genes in DNA repair of A549 cells.

Key words: benzo[a]pyrene, DNA repair genes, XPA gene, XPC gene