Optimization of Fermentation Production Medium of Glutathione by Saccharomyces cerevisiae Using Response Surface Methodology

Abstract

Abstract: The Box-Benhnken’s central composite design and response surface methodology were applied to optimize the medium composition of the mutant Saccharomyces cerevisiae for producing glutathione in this study. The results showed that the glutathione yield and biomass are 120 mg/L and 11.02 g/L respectively,increasing by 71.36% and 39.67%,under the conditions of 38 g/L glucose,6.3 g/L(NH4) 2SO4 and 1.9 g/L L-Cys·HCl being added to 10°Be′ malt extract .

Key words: glutathione, fermentation, response surface methodology, central composite design

ZHENG Li-Xue, LIN Xiao, ZHU Yi-Bo, QI Bin. Optimization of Fermentation Production Medium of Glutathione by Saccharomyces cerevisiae Using Response Surface Methodology[J]. FOOD SCIENCE, 2008, 29(8): 456-459.

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Optimization of Fermentation Production Medium of Glutathione by Saccharomyces cerevisiae Using Response Surface Methodology

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