Abstract:

Contents of flavor nucleotides such as cytidine monophosphate (CMP), uridine monophosphate (UMP), guanosine monophosphate (GMP), inosine monophosphate (IMP) and adenosine monophosphate (AMP) in Penaeus chinensis meat were determined by reverse-phase high performance liquid chromatography（RP-HPLC）. The detection was carried out on an Agela Alasil- C18 column at the wavelength of 260 nm with the mobile phase of CH3OH-0.05 mol/L KH2PO4 solution (1:1, V/V) at 0.8 ml/min. Five linear regression equations were obtained in the range of 0.00 to 0.10 mg/ml and the determination coefficients ranged from 0.9902 to 1.0000. The average spike recoveries of five flavor nucleotides were from 99.96% to 100.34% (n = 3) and the coefficients of variation were in the range from 0.040% to 0.39%. The contents of the above five flavor nucleotides in Penaeus chinensis meat were 18.86 ± 0.28, 0.95 ± 0.13, 44.35 ± 0.81, 50.79 ± 0.57, 139.96 ± 5.15 mg/100 g, respectively.

Key words: Penaeus chinensis, flavor nucleotides, reverse-phase high performance liquid chromatography (RP-HPLC)