Abstract:

Essential oil was extracted from wild Phlomis umbrosa seeds by steam distillation. The chemical components of the essential oil were separated and structurally identified using gas chromatography-mass spectrometry, and the relative contents of the components were determined through peak-area normalization method. The results showed that 56 components exist in essential oil, among which, the major components are α-linalool (13.38%), caryophyllene oxide (11.21%), 1-octen-3-ol (8.38%), phthalic acid, diisobutyl ester (8.22%) and linolenic acid, ethyl ester (6.21%), etc. The essential oil has obvious hydroxyl radical-scavenging effect, and inhibitory and inactivation effects against 8 standard bacteria, especially against Escherichia coli ATCC25922 and Pneumococcus 32201.

Key words: Phlomis umbrosa Turcz, essential oil, gas chromatography-mass spectrometry, antioxidant activity, antimicrobial activity