A Simple Method for Detecting Quorum Sensing Inhibitor in Gram-negative Bacteria

doi:10.7506/spkx1002-6630-200904027

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (4): 142-144.doi: 10.7506/spkx1002-6630-200904027

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A Simple Method for Detecting Quorum Sensing Inhibitor in Gram-negative Bacteria

QI Guo-hong1，DONG Ming-sheng2,*，WANG Sui-lou1

(1. College of Pharmacy, China Pharmaceutical University, Nanjing 210009, China；
2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China)

Received:2008-01-15 Revised:2008-07-11 Online:2009-02-15 Published:2010-12-29
Contact: QI Guo-hong1， E-mail:qghbox@163.com

Abstract

Abstract:

Many Gram-negative bacteria regulate the expression of some physiological traits in quorum sensing (QS) mediated by N-acyl-homoserine lactones (AHLs). Quorum sensing inhibitor (QSI)of AHL antagonists can block or attenuate the expression of characteristic associated with QS. Based on the above inhibitory principle, this study developed an agar plate and TLC assay to detect the antagonists for N-acyl-homoserine in microorganism. This new method is simple, inexpensive and easy to application.

Key words: quorum sensing, quorum sensing inhibitor, n-acyl-homoserine lactones, detection

CLC Number:

Q938

QI Guo-hong1，DONG Ming-sheng2,*，WANG Sui-lou1. A Simple Method for Detecting Quorum Sensing Inhibitor in Gram-negative Bacteria[J]. FOOD SCIENCE, 2009, 30(4): 142-144.

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A Simple Method for Detecting Quorum Sensing Inhibitor in Gram-negative Bacteria

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