FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (6 ): 128-131.doi: 10.7506/spkx1002-6630-200906026

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Separation of ATP from Waste Beer Yeast Hydrolysate by SD3 Ion Exchange Resin

QIAO De-yang,GAN Dan   

  1. (Department of Chemical Engineering, Xuzhou College of Industrial Technology, Xuzhou 221140, China)
  • Received:2008-12-30 Online:2009-03-15 Published:2010-12-29
  • Contact: QIAO De-yang E-mail:qiaodeyang@sohu.com

Abstract:

The dynamic adsorption and desorption of SD3 ion exchange resin to ATP were studied. The results showed that the optimum adsorption conditions of ATP are as follows: pH of waste beer yeast hydrolysate 2.0, its concentration 1.0 mg/ml, its flow rate 8.25 g/L and diameter to height ratio of column 10 (80 g of wet resin filled into the column). The optimum eluent for removing impurity is HCl solution at pH 1.5 containing 0.05 mol/L NaCl. And the optimum elution conditions of ATP are as follows: taking NaOH solution at pH 12.0 containing 0.1 mol/L NaCl as eluent and its flow rate 0.5 ml/min.

Key words: ATP, ion exchange resin, separation

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